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The female Great Horned Owl incubates the eggs alone until hatch. The male owl provides her with prey during this time. In this clip, she begins "squawking", which is a food solicitation call to the male, then flies off. You can continue to hear her squawking in the background (and him hooting) while the eggs are untended. Nearby, a Northern Cardinal sings near-continously in the foreground, American Crows caw in the distance, and a Carolina Wren sings below. She returns soon after first light and settles in for another day of incubation.